Composite

Part:BBa_K4613508:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


YF1-Motif_58.16-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 581
    Illegal NgoMIV site found at 653
    Illegal NgoMIV site found at 743
    Illegal NgoMIV site found at 761
    Illegal NgoMIV site found at 1273
    Illegal NgoMIV site found at 1566
    Illegal NgoMIV site found at 1660
    Illegal AgeI site found at 295
    Illegal AgeI site found at 1441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1330
    Illegal BsaI.rc site found at 194
    Illegal BsaI.rc site found at 2818


Design Notes

Because of the delay of gene systhesis by GENERAL BIOL company, we didn't conduct any wet lab experiments about YF1-Motif_58.16-GFP.


Source

NAU-CHINA

References